In this study, we sought to fabricate a type of anti-HER2 ICG-DOX-loaded polyethyleneimine (PEI)-coated perfluorocarbon (PFC) double nanoemulsions (HIDPPDNEs) to explore the potential of co-administration of photo- and chemotherapy for HER2-overexpressing breast cancer cells. The gene discussed is ERBB2; the disease is breast cancer.