Two out of the five HLHS patients (HLHS3 and HLHS) harboured two and four variants in NOTCH1 and NOTCH4 genes, respectively; one patient (HLHS1) harboured two different variants in NOTCH4 gene and the remaining two patients (HLHS2 and HLHS6) harboured single variants in the NOTCH2 and NOTCH3 genes. Here, NOTCH4 is linked to hypoplastic left heart syndrome.