From this analysis, we observed that three of the HLHS patients (HLHS3, HLHS6 and HLHS) harboured at least one deleterious variant in NOTCH signaling receptors (NOTCH1, NOTCH3 and NOTCH4) genes (Table 3) despite these variants not being identical in each patient. The gene discussed is NOTCH4; the disease is hypoplastic left heart syndrome.