Reverse transcription-PCR (RT-PCR) and quantitative real-time RT-PCR (qRT-PCR) displayed that the mRNA of ASIC1 and ASIC3 in pancreatic cancer cell lines (PANC-1, SW1990, BxPC-3 and AsPC-1) significantly increased compared with normal pancreatic ductal cells (HPDE) (Figure 1a). Here, ASIC1 is linked to familial pancreatic carcinoma.