RHOA and Familial prostate cancer: On the contrary, other study has shown that depletion of RhoA with RNAi leads to the elongated and protrusive phenotype in PC3 prostate cancer cells and MDA-MB-231 breast cancer cells, and therefore correlates with an increase in invasive behavior.46 Nevertheless, these variable consequences of RhoA imply that the role of RhoA for cell migration, invasion and EMT is heterogeneous and has a strong dependence on cell background.47