Therefore, we examined the functions of hCINAP in regulating the metabolism, ROS level and cell death of normal colon stem cell using organoid by isolating colon crypts from the adjacent tissue of colon cancer patients (Supplementary Fig. 5b), CRCNSCs (colorectal cancer non-stem cells, CD133-negative CRC cells selected by FACS) and CRCSCs (CD133-positive CRC cells). Here, PROM1 is linked to malignant colon neoplasm.