Inhibition of CRCSC LDHA activity by hCINAP RNAi depletion markedly attenuated the extracellular acidification rate (ECAR), self-renewal, resistance to conventional chemotherapeutics, and tumour formation in vivo and in vitro, indicating that the Warburg effect is beneficial for CRCSCs because it confers a metabolic advantage and facilitates adaption in an adverse environment. Here, LDHA is linked to neoplasm.