To further directly observe and confirm the correlation of REST expression and Akt–mTOR and Wnt-β-catenin pathways in the prion disease model, p-Akt (Ser473), p-mTOR (Ser2448), p-β-catenin (Ser522), and p-GSK3β (Ser9) levels were quantified in PrP106-126-treated PCCN by immunofluorescence and expressed as percent of the HA-vector-transfected, untreated negative control. Here, AKT1 is linked to prion disease.