Furthermore, Foxo4 only reversed the upregulation of MC5R and Leptin (P<0.05), and the downregulation of Foxo4 and IL-6 by αMSH in mRNA level (P<0.05) (Figure 3H), suggesting that αMSH may inhibit adipocyte inflammation partly via Foxo4 transcriptional regulation. This evidence concerns the gene FOXO4 and inflammation.