Additionally, they showed lower predicted pathogenicity scores than confirmed disease-associated mutations and 45% of them also showed similar substitutions in other primates, including very closely related species, like great apes and other species of the genus Homo. This was the case of the m.3496G>T (A64S), affecting mt-ND1 of Japanese families and associated with LHON [79]. This evidence concerns the gene MT-ND1 and Leber hereditary optic neuropathy.