It has previously been demonstrated that GPC3 was over-expressed in human hepatocellular carcinoma measured by cDNA microarrays, and GPC3 protein was found in serum from 40% of patients with hepatocellular carcinoma, but not in serum from patients with liver cirrhosis, chronic hepatitis, and healthy donors [4]. Here, GPC3 is linked to hepatocellular carcinoma.