Furthermore, total-titin phosphorylation (by ProQ Diamond/Sypro Ruby staining) was unaltered in another set of explanted human DCM hearts, while hypertrophic cardiomyopathy (HCM) hearts showed modest hypo-phosphorylation of titin (Kötter et al. 2013). This evidence concerns the gene TTN and familial dilated cardiomyopathy.