Of the 17 loci robustly associated with major depression in Europeans,13 two were nominally (P<0.1) associated with major depression in our Australian data set, SNP rs10514299 between TMEM161B and MEF2C (allele T: odds ratio (OR)=1.10, 95% CI=1.03–1.17; P=0.006) and SNP rs11209948 near NEGR1 (allele T: OR=1.07, 95% CI=1.01–1.12; P=0.05). This evidence concerns the gene TMEM161B and major depressive disorder.