In this study, we explore the potential of a novel real time (RT)‐qPCR assay for measuring p16 mRNA expression to improve grading of CIN lesions in a set of formalin‐fixed paraffin‐embedded (FFPE) cervical biopsies from a UK colposcopy referral population.22 As a comparison of performance, we also used p16 IHC staining graded by both an expert histopathologist (Visual IHC) and analysed as an H‐Score by an automated digital scanner module (Digital IHC). This evidence concerns the gene CDKN2A and cervical squamous intraepithelial neoplasia.