SMO and lung cancer: To examine the expression profile of SHH signaling components for identification of roles of SHH pathway signaling in human lung cancer tissues, we performed both RT-PCR and immunoblotting and found that endogenous mRNA levels of SHH, SMO and GLI1 are significant higher except for SHH in sample #3 in all five human lung cancers relative to paired normal lung tissues (Fig. 1A).