In contrast, the phosphorylation of AKT and ERK1/2 in response to amino acids in C1 knocked down breast cancer cell lines MDA-MB-231 (Figure 5G), MCF-7 (Figure 6C), and MDA-MB-435s (Figure 6F) cells was similar to that of the control cells expressing scramble shRNA. The gene discussed is AKT1; the disease is breast carcinoma.