In addition, use of sub-clones of these cell lines, that may have acquired different SNPs, may also account for differences in their responses due to altered BET protein function.16, 17 However, it is important to note that JQ1 has been reported to be effective in NUT-midline carcinoma without altering c-myc expression,40 which is similar to our findings from RNA-Seq analysis of JQ1 treated NET cell lines, that did not detect any changes in p53 or c-myc expression. This evidence concerns the gene TP53 and nut midline carcinoma.