To investigate the value of blood HMGB1 in predicting epilepsy development (Figure 2 and Supplemental Figure 5A), we used the electrically induced SE model (also used in Figure 1) in which all rats develop epilepsy to test whether blood HMGB1 levels change before the onset of the spontaneous seizures independently of ongoing seizure activity. The gene discussed is HMGB1; the disease is epilepsy.