To address this, we first seup an in vitro three cell killing assay, where OVA-loaded murine lymphoma EL-4 cells were co-cultured with CD8+ Tc prepared from OT-I T cell receptor (TCR) transgenic mice in the presence of resting naive (rTreg) or activated effector (aTreg) Tregs. The gene discussed is CD8A; the disease is lymphoma.