CD8A and neoplasm: For in vivo experiments, Pmel-1 CD8+ Tc expressing CatCh were adoptively transferred into C57BL/6 mice bearing subcutaneous B16 tumours established for 7 days, followed by vaccination with mgp10025–33 (EGSRNQDWL) or hgp10025–33 peptide (Fig. 4b and Supplementary Fig. 5B).