PMEL and melanoma: In this study, we used the Pmel-1 TCR transgenic mouse model, a well characterized mouse tumour model for low-immunogenicity, which expresses the Vα1Vβ13 TCR that recognizes an H-2Db-restricted epitope corresponding to amino acids 25–33 of mouse gp100 (mgp100) on the B16 melanoma cells36.