Furthermore, antigen-specific vaccination with a self-antigen, mgp100 or a mgp100 altered peptide ligand (for example, human gp100; hgp100) is not sufficient to improve the antitumour effects of adoptively transferred Pmel-1 T cells against B16 tumours due to an increase in the local CD4+FoxP3+ Treg cell population36, 37. Here, PMEL is linked to neoplasm.