To assess the pathological role of Alox5 in MLL-rearranged AML, we cloned Alox5 CDS into MSCV-PIG retroviral vector, and then co-transduced MSCVneo-MLL-AF9 (MA9) with MSCV-PIG-Alox5 (Alox5) or MSCV-PIG (Ctrl) into mouse BM progenitor cells for in vitro colony-forming/replating assays. Here, KMT2A is linked to acute myeloid leukemia.