We isolated and cultured KCs from WT, NLRP3−/− and TXNIP−/− mice in the absence or presence of PA, an established NLRP3 activator, to evaluate the protective role of TXNIP on the progression of NAFLD in vitro by analyzing the expressions of NLRP3 inflammasome components and IL-1β secretion in KCs. Here, IL1B is linked to metabolic dysfunction-associated steatotic liver disease.