Immunohistochemical staining showed that the numbers of F4/80-, NLRP3- and TXNIP-positive cells were similar between the normal and NAFL groups (Figure 1C II vs I, V vs IV, VIII vs VII), whereas more F4/80-, NLRP3- and TXNIP-positive cells were observed in the NASH group as indicated by arrows than in the NAFL group (Figure 1C III, VI, IX, X-XII). This evidence concerns the gene NLRP3 and non-alcoholic fatty liver.