Furthermore, we explored the important mechanisms by which TXNIP and NLRP3 inflammasome regulate inflammatory responses during NAFLD progression, and we found that the NLRP3 inflammasome and IL-1β secretion pathway in KCs were mainly activated during the progression from NAFL to NASH in WT mice, rather than from normal liver to NAFL. This evidence concerns the gene IL1B and non-alcoholic fatty liver.