However, our results both in vivo and in vitro showed that TXNIP deficiency exacerbated NAFL and resulted in NASH formation, and that NLRP3 inflammasome activation and IL-1β secretion in KCs were not blocked but were enhanced in the absence of TXNIP, thus suggesting that TXNIP suppresses the expression and function of NLRP3 inflammasome by binding to NLRP3. This evidence concerns the gene IL1B and metabolic dysfunction-associated steatohepatitis.