In the present study, we found that FFA, as a mechanistic activator of NLRP3 inflammasome, activated NLRP3 inflammasome and induced IL-1β secretion in KCs and that the expression of NLRP3 increased in liver tissues and was accompanied by elevated levels of FFA and IL-1β in serum of NASH patients and MCD induced mice model. Here, IL1B is linked to metabolic dysfunction-associated steatohepatitis.