Furthermore, we explored the important mechanisms by which TXNIP and NLRP3 inflammasome regulate inflammatory responses during NAFLD progression, and we found that the NLRP3 inflammasome and IL-1β secretion pathway in KCs were mainly activated during the progression from NAFL to NASH in WT mice, rather than from normal liver to NAFL. The gene discussed is IL1B; the disease is metabolic dysfunction-associated steatohepatitis.