Immunohistochemical staining revealed a greater number of F4/80-, NLRP3- and TXNIP-positive cells in the livers of WT mice in NASH group (arrows) than in normal and NAFL groups, whereas there were no significant differences in these cell populations between NAFL and normal groups (Figure 2A). This evidence concerns the gene TXNIP and metabolic dysfunction-associated steatohepatitis.