Bioinformatic analysis of genes of interest against TCGA RNA sequencing data proposed TRIM28, VIM, NAP1L1 and DPYSL2 as promising “glioma versus reference” biomarkers, and suggested CRMP1, NAP1L1, NUCL, ACTB and VIM for discrimination between GBM and LGG. The gene discussed is ACTB; the disease is glioma.