In order to study the respective roles of BRAF and CRAF in melanoma growth after tumour initiation, we generated a second NRAS-mutated melanoma model in which Raf gene ablation is controlled in a spatiotemporal manner by using the Tyr::CreERT2/o transgene expressing a tamoxifen-inducible Cre recombinase29. Here, RAF1 is linked to melanoma.