To test if these cellular and molecular effects observed after DAXX knockdown were PTEN dependent, DAXX expression was knocked down in glioma cell lines and GBM-spheres that express wild-type PTEN. No significant changes in total protein levels of CYCLIN-D1, MYC, FOS and BCL2 were detected after DAXX knockdown in PTEN-WT cells compared with shControl/PTEN-WT cells (Fig. 3b and Supplementary Fig. 7a,e). This evidence concerns the gene CCND1 and glioma.