To investigate if DAXX disruption can restore H3.3 deposition to the oncogene promoters interrogated in Fig. 2, and consequently suppresses their expression in PTEN-deficient cells, lentiviral-encoded shRNAs targeting DAXX were used to generate stable DAXX-knockdown (Daxx-kd) in glioma cell lines and GBM-PDX neurospheres. The gene discussed is PTEN; the disease is glioma.