INS and Hyperglycemia: More relevant is a recent study measuring endogenous insulin and c-peptide levels during graded hyperglycemia (dextrose infusion to a maximum BGL of 18 mmol/l) following a 4-h isoglycemic clamp with either saline (no insulin, sham) or insulin (2 milliunits/kg/min) in healthy volunteers and in subjects with T2DM [7].