To determine the impact of CIN activity on daily home-cage feeding bouts, mice expressing Gq-linked (n = 10), Gs-linked (n = 14), and Gi-linked (n = 10) linked DREADDs and MCY only (n = 15) in NAc-CINs were injected with either CNO or vehicle (VEH) 30 min before the start of the active phase, when mice normally show a significant increase in food intake. Here, GNAI1 is linked to cervical squamous intraepithelial neoplasia.