Therefore, we generated Treg-replete tumor-bearing mice by subcutaneous thigh injection of OVA-expressing B16 melanoma (0.5×106 cells per mouse) into DEREG (Foxp3-DTR) mice that express diphtheria toxin (DT) receptor-enhanced green fluorescent protein under control of the Foxp3 promoter. The gene discussed is FOXP3; the disease is neoplasm.