MAP1LC3B and malignant colon neoplasm: Immunoblot assays against LC3B also showed a significant elevation of the LC3B-II/LC3B-I protein in the 50, 100, 200 and 400 μM peiminine-treated HCT-116 (as comparison), HeLa and MEF cells, suggesting that peiminine induces autophagy in colon cancer cells as well as other cells (Figure 1F).