In order to understand the functional implications of the simultaneous increase in expression of positive (PDLP1, GSL4) and negative (BG3, ANKs) regulators of callose deposition on CBSV infection, we stained callose in leaf tissue from 60444 and KBH 2006/18 at 60 dag (Fig. 2c). This evidence concerns the gene ANKRD46 and infection.