Overexpressed EGFP-Rab20, which has been shown to associate with early latex bead phagosomes (Pei et al., 2014), associated with RFP-Mtb phagosomes very early but unexpectedly remained associated for 24 hr after infection (Figures 1A, S1E, and S1F; Movie S2). The gene discussed is RAB20; the disease is infection.