We then knocked down CSIG in the HCC cell lines HepG2 and SMMC7721 as well as liver immortalized L02, and the results showed that the expression of NOLC1 was increased after CSIG knockdown (Fig. 6C), which indicated that the down‐regulation of NOLC1 in HCC was correlated with the higher expression of CSIG in HCC. The gene discussed is NOLC1; the disease is hepatocellular carcinoma.