IGF1R and colorectal carcinoma: miR-143 has been reported to target and repress KRAS, IGF1R, Bcl-2, and HK2 expression in CRC.23, 24, 25, 26, 27 Western blot assays revealed that the expression levels of these genes were dysregulated in CCD841 cells compared to SW620 cells (Supplementary Figure S6) and that forced expression of miR-143 triggered a significant silencing effect on the expression of these genes in SW620 cells, confirming that they are targets of miR-143 (Figure 6f).