We examined the influence of breast cancer cells on glycolysis of myeloid cells through coculture of bone marrow monocytes and neutrophils with 4T1 cells or primary mouse fat pad mammary epithelial cells (MECs) and found that both monocytes and neutrophils encountering 4T1 cancer cells showed higher levels of mRNAs of glycolytic enzymes, especially Pfkl, Pfkp and Eno2, in comparison with MEC-experienced cells (Figure 3a). This evidence concerns the gene ENO2 and breast cancer.