The best practices include 11 care strategies to achieve faster DTN: emergency medical services pre-notification to hospitals about stroke patients, hospital-specific stroke protocols, a rapid stroke identification and notification system, a single-call stroke activation system, direct to CT protocol, rapid transportation to imaging, rapid interpretation of imaging, rapid protocols for laboratory testing, pre-mixing of r-tPA in patients with high likelihood of stroke, a team-based approach, and a prompt feedback system. This evidence concerns the gene PLAT and stroke disorder.