TP53 and neoplasm: In addition, 30 sig-neg-AFD mutations were observed on 6 genes both annotated as oncogenes and TSGs while the majority of them (n = 23) were observed in TP53. Moreover, for SNVs, the significant enrichment of sig-neg-AFD mutations were observed in the NMD-sensitive nonsense SNVs compared to other classes including the NMD-insensitive nonsense SNVs across the five tumor types (Fig. 2(f); P value 1.96 × 10−214; Fisher’s exact test).