SOD1 and amyotrophic lateral sclerosis: At this individually defined disease onset, we intravenously administered 1.2 × 1012 vg of AAV‐D7 into each given male ALS mouse to perform survival analysis according to the guideline for preclinical ALS studies (Ludolph et al, 2010), which requires a minimum of 12 mice of a single gender and of comparable hSOD1‐G93A transgene copy number for each test or control group.