In our histological analyses, we noticed that the Pten knockout offspring of HF/HS-fed dams had more prostatic cells undergoing mitosis than did Pten knockout offspring of Chow-fed dams (Fig 3C, black arrows) (Chow 6[3–15] vs. HF/HS 9[8–18], median [range], p<0.01 (Fig 3D)). This evidence concerns the gene PTEN and hydrops fetalis.