In this model, we detected an increase in both phospho-Akt (Fig 5C and 5D) and total Akt (Fig 5G and 5H) with the loss of Pten (Fig 5K and 5L) in Pten KO mice but there was no additive effect of the HF/HS diet on phospho-Akt and Akt expression (Fig 5D and 5H versus Fig 5C and 5G). The gene discussed is AKT1; the disease is hydrops fetalis.