Furthermore, real-time PCR analysis showed that the expression of Wnt target genes such as CTNNB1 (β-Catenin), CD44, CD31 and CCND1(CyclinD1) were decreased in gastric cancer cells treated with regorafenib at the concentration of 20μmol/L, but overexpression of CXCR4 increased the mRNA levels of Wnt target genes (Fig 5B, S7 Table). The gene discussed is CCND1; the disease is gastric cancer.