We found that POU2F1 protein was over-expressed in 51.57% (49/95) of the HCCs, but only 25.88% (22 of 85) of the non-HCC tissues were stained positively for POU2F1, and the difference in POU2F1 staining between the HCC and adjacent non-tumor hepatic (N) tissues was statistically significant (P < 0.0001) (Figure 1D). This evidence concerns the gene POU2F1 and hepatocellular carcinoma.