Here, we demonstrated that up-regulation of POU2F1 increased mRNA levels of EMT genes such as Twist1, Snai1, Snai2 and ZEB1 in HCC cells, while silencing of POU2F1 down-regulated the mRNA levels of these genes (Figure 8A and 8B). The gene discussed is SNAI1; the disease is hepatocellular carcinoma.