Continuous infusion of commonly used hypertrophic inducer phenylephrine (70 mg/kg/day) or angiotensin II (1.5 mg/kg/day) to C57 mice via an implantable Alzet osmotic pump for 2 weeks resulted in cardiac hypertrophy, as indicated by an enlargement in heart size and an increased ratio of heart weight/total body weight (Figures 1A–C and Supplementary Figures S1A,B). Here, AGT is linked to cardiac hypertrophy.