Sanger sequencing was conducted with priority to the compound heterozygous variants (c.71 C > T, p.P24L and c.1387-1389delGAG, p.E463- in family 1; c.71 C > T, p.P24L and c.134 G > A, p.W45* in family 2;NM_000026) in ADSL (the only gene associated with seizures and neurodevelopmental delay among the candidate genes list above) for excluding the false positive of WES. This evidence concerns the gene ADSL and Neurodevelopmental delay.