Scatter plots of allele frequencies demonstrate a significant correlation between bulk tumor and tumor EPCAM+CD44+CD49f+ cells somatic variant allele frequencies (R = 0.72, p < 0.001) (Figure 2B), between tumor EPCAM+CD44+CD49f+ cells and lymph node EPCAM+CD44+CD49f+ cells somatic variant allele frequencies (R = 0.71, p < 0.001) (Figure 2C), while bulk lymph nodes demonstrated an absence (allele frequency < 0.10) of the variants present in the primary tumor or EPCAM+CD44+CD49f+ cells (Supplementary Figure 2A and Supplementary Figure 2B). This evidence concerns the gene ITGA6 and neoplasm.