In vivo studies further revealed that tumor uptake of 64Cu-NOTA-rh-HGF was rapidly and clearly visible at 0.5 h post-injection (p.i.)and peaked at 9 h p.i. (6.7 ± 1.8%ID/g) in U87-MG xenografts in mice; however, it was significantly lower in MDA-MB-231 xenografts in mice (1.8 ± 0.6%ID/g at 9 h p.i.), with consistency between PET images and biodistribution data in both models (Fig. 2). Here, HGF is linked to neoplasm.