CD4 and acute graft versus host disease: In the univariate analysis, DSAs (MFI ≥ 1000, P = 0.076), grade II–IV acute GVHD (P = 0.033), infused CD4+ (P = 0.024) and CD3+ cells (P = 0.088), donor-recipient relationship (P = 0.097), and disease status at the transplantation (P = 0.029) were associated with TRM.