We previously obtained cryopreserved single-cell suspensions of splenic tissue from five patients with chronic untreated HIV infection (23), from which CD3+CD4+CD19− cells were sorted into naïve CD45RA+ICOS− and resting memory cell (CD45RA−ICOS−), and two subsets of Tfh defined as (i) CXCR5int+ Tfh (CD45RA−ICOS+PD-1hi+CXCR5int+) and (ii) CXCR5hi+ GC Tfh (CD45RA−ICOS+PD-1hi+CXCR5hi+), as shown in Figure S2 in Supplementary Material. The gene discussed is CD4; the disease is HIV infectious disease.