HMGB1 and infection: Quantitative-RT-PCR results indicated that, in the early 12 h after infection, HMGB1 transcription began to rise, which is similar to the test in vitro using IFN-γ treated ANA1 cells (Figure 1A), but then decreased gradually to below the basal level, and there were no upward trends again in the detecting period (Figure 1B).