As bacterial TIR proteins down‐modulate NF‐κB activation (Newman et al, 2006; Cirl et al, 2008; Salcedo et al, 2008, 2013; Spear et al, 2012; Askarian et al, 2014; Zou et al, 2014), we infected the lung carcinoma epithelial cell line A549, a well‐established cellular model for Pseudomonas infection and analysed NF‐κB translocation into the nucleus after one hour of infection by confocal microscopy. Here, NFKB1 is linked to Pseudomonas infection.