The results demonstrated that serum levels of the pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) were dramatically increased in mice with sepsis 12 h after CLP surgery compared to mice with sham surgery (ANOVA: F(4,29) = 40.17, P < 0.0001; F(4,29) = 97.86, P < 0.0001; F(4,29) = 36.25, P < 0.0001, respectively). This evidence concerns the gene IL1B and Sepsis.