To further observe whether the deficiency of Shp2 affected the polarization of asthma-related TH subsets, we detected the ratios of TH2 (stained by FITC-CD4 and APC-IL-4) and TH17 (stained by FITC-CD4 and PE-IL-17A) in the lung tissue suspension. Here, CD4 is linked to asthma.