SIAH1 and gastric adenocarcinoma: For this, the highly adherent gastric adenocarcinoma cell AGS was preferred over the partially adherent MKN45 cells (poorly-differentiated gastric adenocarcinoma cells) since MKN45 cells partly grow in clumps.35 pcDNA3.1+ or Siah1 stably-expressing AGS cells were grown in monolayer, a wound was marked and cells were incubated in the presence or absence of H. pylori for various time periods.