In summary, we were able to show that serum collected from young men after acute exercise resulted in significant inhibition of lung cancer cell survival and proliferation in vitro which was associated with significant inhibition of phosphorylation/activation of Akt, mTOR, p70 S6K, and Erk1/2 levels compared to cells treated with serum taken pre-exercise. This evidence concerns the gene RPS6KB1 and lung cancer.