Of note, we saw no evidence of α-synuclein accumulation or enhanced oligomer (or fibril) formation at 24 day post-reovirus-T3D inoculation in skull sections of those mice that had survived their encephalitis; however, as discussed above, the holocranohistochemistry technique may not be sensitive enough to detect mild-to-moderate changes of this long-lived protein (Fishbein et al. 2014) by microscopy alone, and the time course for any dysregulation to occur may have been too short (i.e., between the start of encephalitis at 9 dpi and euthanasia at 24 dpi). This evidence concerns the gene SNCA and encephalitis.