Interestingly, IFN-γ production dramatically increased in Tri-CD8 T cells, compared with Single-CD8 T cells, suggesting that the response of Tri-CD8 T cells to certain tumor antigens can be stronger than that of Single-CD8 T cells (Figure 2A), which may miss or react weakly to a specific subset of leukemic cells. This evidence concerns the gene CD8A and neoplasm.